miR-145 suppresses epithelial-mesenchymal transition by targeting stem cells in Ewing sarcoma cells

Objectives We aimed to examine the performance of stem cell markers and epithelial-mesenchymal transition (EMT) process in miR-145 transfected EWS cells (TC71, TC106). Methods were utilized for functional analysis mir-145. Proliferation, migration, invasion soft agar colony assay performed observe alterations migration behavior cells. Caspase was used investigate underlying reasons proliferative inhibition whichmiR-145 is overexpressed. QRT-PCR determine role EMT transcription mir-145 targeted genes, KLF4, SOX2 OCT4 expression levels. Results The has been shown be down-regulated EWS. overexpression caused proliferation reduced through induction apoptosis. Mir-145 suppresses capacity SOX2, KLF4 Conclusion This first time literature we have deregulation inhibits by targeting properties leading tumor growth metastasis TC71 TC106 Based on these results, propose that miR-145, as an important regulator carries crucial roles tumorigenesis (Tab. 1, Fig. 4, Ref. 26).